LOCUS       sequence-118953-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArf6 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARF6
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(562..586)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(639..657)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      653..700
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tggggaaggt gctatccaaa atcttcggga
       61 acaaggaaat gcggatcctc atgttgggcc tggacgcggc cggcaagaca acaatcctgt
      121 acaagttgaa gctgggccag tcggtgacca ccattcccac tgtgggtttc aacgtggaga
      181 cggtgactta caaaaatgtc aagttcaacg tatgggatgt gggcggccag gacaagatcc
      241 ggccgctctg gcggcattac tacactggga cccaaggtct catcttcgta gtggactgcg
      301 ccgaccgcga ccgcatcgat gaggctcgcc aggagctgca ccgcattatc aatgaccggg
      361 agatgaggga cgccataatc ctcatcttcg ccaacaagca ggacctgccc gatgccatga
      421 aaccccacga gatccaggag aaactgggcc tgacccggat tcgggacagg aactggtatg
      481 tgcagccctc ctgtgccacc tcaggggacg gactctatga ggggctcaca tggttaacct
      541 ctaactacaa atcttaatag gacccagctt tcttgtacaa agtggtgatg atccggctgc
      601 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      661 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      721 cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
      781 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacggggt
      841 gcgcatagaa attgcatcaa acgcatatag cgctagcagc acgccatagt gactggcgaa
      901 tgctgtcgga atggacaata tccc
//
