LOCUS       sequence-118956-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl2 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL2
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(589..613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(666..684)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      680..727
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tggggctcct gaccattctg aagaagatga
       61 agcagaaaga gcgggagctg cgactgctca tgcttggcct ggacaatgct ggaaagacaa
      121 ccatcctgaa gaagttcaat ggggaggaca tcgacaccat ctccccaacg ctgggcttca
      181 acatcaagac cctggagcac cgaggattca agctgaacat ctgggatgtg ggtggccaga
      241 agtccctgcg gtcctactgg cggaactact ttgagagcac cgatggcctc atctgggtag
      301 tggacagcgc agaccgccag cgcatgcagg actgccagcg ggagctccag agcctgctgg
      361 tggaggagcg cctggccgga gcaaccctcc tcatctttgc taataagcag gacctgcctg
      421 gagcactgtc ctctaacgcc atccgcgagg ccctggagct ggactccatc cgcagccacc
      481 actggtgcat ccagggctgc agcgccgtca ccggggagaa cctgctgccg ggcatcgact
      541 ggctcctgga tgacatttcc agccgcattt tcacagctga ctgataggac ccagctttct
      601 tgtacaaagt ggtgatgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      661 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      721 ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga
      781 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cgg
//
