LOCUS       sequence-118959-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl3 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL3
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(583..607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(660..678)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      674..721
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggcttgct ctcaattttg cgcaagttga
       61 aaagtgcacc agaccaggag gtgagaatac ttctcctggg cttggataat gctggcaaga
      121 ccactcttct gaagcagctt gcatctgaag acatcagcca catcacacct acacagggtt
      181 tcaacatcaa aagtgtacaa tcacaaggtt ttaaactgaa tgtatgggac attggtggac
      241 agaggaaaat cagaccatac tggaagaatt attttgaaaa taccgatatt cttatatatg
      301 taatcgacag tgcagacaga aaaagatttg aagagacggg tcaggaacta gcggaattac
      361 tggaggaaga aaaactaagt tgtgtgccag tgctcatctt tgctaataag caggatttgc
      421 tcacagcagc ccctgcctct gaaattgcag aaggactgaa cctgcatacc atccgcgacc
      481 gagtctggca gatccagtct tgctcagctc tcacaggaga gggcgttcag gatggcatga
      541 actgggtctg caaaaatgtc aatgcaaaga agaaataata ggacccagct ttcttgtaca
      601 aagtggtgat gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      661 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      721 gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt
      781 agtcgatagt ggctccaagt agcgaagcga gcaggactgg gccggcgggc caaagcggtc
      841 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
      901 cacgcccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggccc
//
