LOCUS       sequence-118960-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein gamma-11 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-11
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          138..270
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     253..272
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        315..333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      356..365
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agaagttggt
      121 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
      181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      241 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt
      301 aaggctagag tacttaatac gactcactat aggctagcca gctggcgcgg atatcgccac
      361 catgggatcc gaaatcggta caggcttccc cttcgacccc cattatgtgg aagtcctggg
      421 cgagcgtatg cactacgtcg atgttggacc gcgggatggc acgcctgtgc tgttcctgca
      481 cggtaacccg acctcgtcct acctgtggcg caacatcatc ccgcatgtag caccgagtca
      541 tcggtgcatt gctccagacc tgatcgggat gggaaaatcg gacaaaccag acctcgatta
      601 tttcttcgac gaccacgtcc gctacctcga tgccttcatc gaagccttgg gtttggaaga
      661 ggtcgtcctg gtcatccacg actggggctc agctctcgga ttccactggg ccaagcgcaa
      721 tccggaacgg gtcaaaggta ttgcatgtat ggaattcatc cggcctatcc cgacgtggga
      781 cgaatggcca gaattcgccc gtgagacctt ccaggccttc cggaccgccg acgtcgg
//
