LOCUS       sequence-118962-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl4 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL4
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(637..661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(714..732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      728..775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tggggaatgg gctgtcagac cagacttcta
       61 tcctgtccaa cctgccttca tttcagtctt tccacattgt tattctgggt ttggactgtg
      121 ctggaaagac aactgtctta tacaggctgc agttcaatga atttgtaaat accgtaccta
      181 ccaaaggatt taacactgag aaaattaagg taaccttggg aaattctaaa acagtcactt
      241 ttcacttctg ggatgtaggt ggtcaggaga aattaaggcc actgtggaag tcatatacca
      301 gatgcacaga tggcattgta tttgttgtgg actctgttga tgtcgaaagg atggaagaag
      361 ccaaaactga acttcacaaa ataactagga tatcagaaaa tcagggagtc cctgtactta
      421 tagttgctaa caaacaagat ttgaggaact cattgtcact ttcagaaatt gagaaattgt
      481 tagcaatggg tgaactgagc tcatcaactc cttggcattt gcagcctacc tgtgcaatca
      541 taggagatgg cctaaaggaa ggacttgaga aactacatga tatgatcatt aaaagaagaa
      601 aaatgttgcg gcaacagaaa aagaaaagat gataggaccc agctttcttg tacaaagtgg
      661 tgatgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      781 aggaggaact atatccggat atccacagga cggggtgtgg tcgccatgat cgcgtagtcg
      841 atagtggctc caagtagcga agcgagcagg aactgggccg gcggccaaag cggtcggaca
      901 gtggctccga agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg c
//
