LOCUS       sequence-118963-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein gamma-12 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-12
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          131..263
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     246..265
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        308..326
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      349..358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtgaaccgt cagatcacta gaagctttat tgcggtagtt tatcacagtt aaattgctaa
       61 cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt ggtcgtgagg
      121 cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg
      181 cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat
      241 ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct cttaaggcta
      301 gagtacttaa tacgactcac tataggctag ccagctggcg cggatatcgc caccatggga
      361 tccgaaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
      421 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
      481 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
      541 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
      601 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
      661 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
      721 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
      781 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
      841 atcatcgatc agaacgcttt catcgagggt gcgctcccg
//
