LOCUS       sequence-118965-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl5 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL5A
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(574..598)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(651..669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      665..712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggaattct cttcactaga atatggagac
       61 tgttcaatca ccaggagcac aaagttatca ttgttgggtt ggataatgca gggaaaacta
      121 ccattcttta ccaattttct atgaatgaag ttgtacatac atctcctaca ataggaagta
      181 atgtagaaga gatagtgatt aataatacac gtttcctaat gtgggatatt ggtggccaag
      241 aatctcttcg ttcttcctgg aacacttact atactaacac agagtttgta atagttgttg
      301 tggacagtac agacagagag aggatttctg taactagaga agaactctat aaaatgttag
      361 cgcatgagga cctaagaaaa gctggattgc tgatttttgc taataaacaa gatgttaaag
      421 aatgcatgac tgtagcagaa atctcccagt ttttgaagct aacttctatt aaagatcacc
      481 agtggcatat ccaggcatgc tgtgctctaa ctggcgaggg attgtgccaa ggacttgaat
      541 ggatgatgtc acgacttaag attagatgat aggacccagc tttcttgtac aaagtggtga
      601 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      661 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      721 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
      781 tggctccaag tagcgaagcg agca
//
