LOCUS       sequence-118966-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl5b in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL5B
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(574..598)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(651..669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      665..712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tggggctgat cttcgccaaa ctgtggagcc
       61 tcttctgtaa ccaagaacac aaagtaatta tagtgggact ggataatgca gggaaaacca
      121 ccattcttta ccaattctta atgaatgaag tggttcatac ttctccaacc ataggaagca
      181 atgttgaaga aatagttgtg aagaacactc attttcttat gtgggatatt ggtggtcagg
      241 agtctctgcg atcatcctgg aacacatatt actcaaatac agagttcatc attcttgttg
      301 ttgatagcat tgacagggaa cgactagcta ttacaaaaga agaattatac agaatgttgg
      361 ctcatgagga tttacggaag gctgcagtcc ttatctttgc aaataaacag gatatgaaag
      421 ggtgtatgac agcagctgaa atctcgaaat acctcaccct tagttcaatt aaggatcatc
      481 catggcacat tcaatcctgc tgtgctctca caggagaagg gttatgccaa ggtctagagt
      541 ggatgacctc ccggattggt gtgagataat aggacccagc tttcttgtac aaagtggtga
      601 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      661 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      721 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
      781 tggctccaag tagcgaagcg agcaggactg gggcggcggc caaagcgggt cggacagtgc
      841 tccgagaacg ggtgcgcata gaaaattgca tcaacgcata tagcgctagc agcacgccat
      901 agtgactggc gatgctgtcg gaatggacga tatcccgcaa ga
//
