LOCUS       sequence-118970-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This red fluorescent plasma membrane marker consists of the first 
            twenty residues of neuromodulin fused to mCherry. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: mCherry-Mem
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..96
                     /label=MCS
                     /note="multiple cloning site"
     CDS             164..874
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALR
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(309..327)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     577..596
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgctgt gctgtatgag
      121 aagaaccaaa caggttgaaa agaatgatga ggaccaaaag atcatggtga gcaagggcga
      181 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      241 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      301 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      361 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      421 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      481 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      541 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      601 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga ggggcgagat
      661 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      721 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      781 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      841 ctccaccggc ggcatggacg agctgtacaa gtaagc
//
