LOCUS       sequence-118971-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl8 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL8A
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(595..619)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgatcgcttt gttcaacaag ctgctggact
       61 ggttcaaggc cctattctgg aaggaggaga tggagctcac gctggtcggg cttcagtact
      121 cgggcaagac caccttcgtc aacgtgatcg cgtcaggaca gttcaacgag gacatgatcc
      181 ccaccgtggg tttcaacatg cgcaaaatca ccaaagggaa tgtgactatc aagctctggg
      241 acattggggg acagccgcgt ttccgcagca tgtgggagcg ctactgccga ggagtgagcg
      301 ccatcgtgta catggtggat gctgctgacc aggagaagat tgaggcctct aagaacgagc
      361 tccacaacct actggacaaa cctcagctgc agggcatccc ggtcttagtc ctgggtaaca
      421 agcgagacct tccgggagca ttggatgaga aggagctgat tgagaaaatg aatctgtctg
      481 ccatccagga ccgagagatc tgctgctact ccatctcttg caaagaaaag gacaacattg
      541 acatcaccct acagtggctt attcaacact cgaagtcacg gagaagctga taggacccag
      601 ctttcttgta caaagtggtg atgatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg
      781 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
      841 caaagcggtc ggacagtgct ccgagaacgg ggtgcgcata gaaattgcat caacgcatat
      901 agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaa
//
