LOCUS       sequence-118972-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl8b in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL8B
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(595..619)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgctggcgct catctcccgc ctgctggact
       61 ggttccgttc gctcttctgg aaggaagaga tggagctgac gctcgtgggg ctgcagtact
      121 cgggcaagac caccttcgtc aatgtcatcg cgtcaggtca attcagtgaa gatatgatac
      181 ccacagtggg cttcaacatg aggaaggtaa ctaaaggtaa cgtcacaata aagatctggg
      241 acataggagg acaaccccga tttcgaagca tgtgggagcg gtattgcaga ggagtcaatg
      301 ctattgttta catgatagat gctgcagatc gtgaaaagat agaagcttcc cgaaatgagc
      361 tacataatct tctagataaa ccacagttac aaggaattcc agtgctagtg cttggaaaca
      421 agagagatct tcctaatgcc ttggatgaga aacagctaat tgaaaaaatg aatctgtctg
      481 ctattcagga tagagaaatt tgctgctatt caatttcttg caaagaaaag gataatatag
      541 atatcacact tcagtggctt attcagcatt caaaatctag aagaagctga taggacccag
      601 ctttcttgta caaagtggtg atgatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg
      781 ccatgatcgc gtagtcgata gtggctccaa gtagcgaaag cgagcaggac tgggcggcgg
      841 ccaaagcggt cggaca
//
