LOCUS       sequence-118974-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArl14 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARL14
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(613..637)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(690..708)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      704..751
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggttcgct gggttctaaa aatccgcaaa
       61 ccaaacaagc ccaagttctt cttttgggac ttgactcagc tgggaagtct actctccttt
      121 ataaattaaa gcttgctaag gatattacca ccatccctac aataggtttc aatgtggaaa
      181 tgatcgagtt ggaaaggaat ttttcactca cagtctggga tgttggagga caggaaaaaa
      241 tgagaactgt ttggggctgt tactgtgaga acaccgatgg gctggtgtat gttgtggaca
      301 gtacagacaa acagcgactg gaagagtctc agagacagtt tgagcacatt ttgaagaatg
      361 aacacattaa aaatgtgcct gttgttctat tagccaacaa acaagacatg cctggagctc
      421 tgactgctga ggacatcacc agaatgttca aagtgaagaa gctttgcagt gaccggaact
      481 ggtatgtgca accctgctgt gccctcacag gggaggggct ggcccagggg ttcaggaaat
      541 taactggatt tgtgaagagc cacatgaaat caagaggaga cactttggcg ttcttcaagc
      601 agaactgata ggacccagct ttcttgtaca aagtggtgat gatccggctg ctaacaaagc
      661 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      721 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
      781 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
      841 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
      901 attgcatcaa cgcatatagc gctagcagca cgcccatagt gaactggcga tgctgtcgga
      961 atggacgat
//
