LOCUS       sequence-118976-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hSar1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-SAR1
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(631..655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(708..726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      722..769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgtctttcat ctttgagtgg atctacaatg
       61 gcttcagcag tgtgctccag ttcctaggac tgtacaagaa atctggaaaa cttgtattct
      121 taggtttgga taatgcaggc aaaaccactc ttcttcacat gctcaaagat gacagattgg
      181 gccaacatgt tccaacacta catccgacat cagaagagct aacaattgct ggaatgacct
      241 ttacaacttt tgatcttggt gggcacgagc aagcacgtcg cgtttggaaa aattatctcc
      301 cagcaattaa tgggattgtc tttctggtgg actgtgcaga tcattctcgc ctcgtggaat
      361 ccaaagttga gcttaatgct ttaatgactg atgaaacaat atccaatgtg ccaatcctta
      421 tcttgggtaa caaaattgac agaacagatg caatcagtga agaaaaactc cgtgagatat
      481 ttgggcttta tggacagacc acaggaaagg ggaatgtgac cctgaaggag ctgaatgctc
      541 gccccatgga agtgttcatg tgcagtgtgc tcaagaggca aggttacggc gagggtttcc
      601 gctggctctc ccagtatatt gactgatagg acccagcttt cttgtacaaa gtggtgatga
      661 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      721 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      781 aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg
      841 ctccaagtag cgaa
//
