LOCUS       sequence-118979-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hSara in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-SARA
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(631..655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(708..726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      722..769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgtccttcat atttgattgg atttacagtg
       61 gtttcagcag tgtgctacag tttttaggat tatataagaa aactggtaaa ctggtatttc
      121 ttggattgga taatgcagga aaaacaacat tgctacacat gctaaaagat gacagacttg
      181 gacaacatgt cccaacatta catcccactt ccgaagaact gaccattgct ggcatgacgt
      241 ttacaacttt tgatctgggt ggacatgttc aagctcgaag agtgtggaaa aactaccttc
      301 ctgctatcaa tggcattgta tttctggtgg attgtgcaga ccacgaaagg ctgttagagt
      361 caaaagaaga acttgattca ctaatgacag atgaaaccat tgctaatgtg cctatactga
      421 ttcttgggaa taagatcgac agacctgaag ccatcagtga agagaggttg cgagagatgt
      481 ttggtttata tggtcagaca acaggaaagg ggagtatatc tctgaaagaa ctgaatgccc
      541 gacccttaga agttttcatg tgtagtgtgc tcaaaagaca aggttacgga gaaggcttcc
      601 gctggatggc acagtacatt gattaatagg acccagcttt cttgtacaaa gtggtgatga
      661 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      721 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      781 aactatatcc ggatatccac aggacgggtg tgggtcgcca tgatcgcgta
//
