LOCUS       sequence-118980-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf1 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF1-GFP
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(644..668)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(700..719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(701..719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(764..969)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(793..813)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtccttctgg ttccggagct gattggacag ccagtccagt
      121 ccttcataga gcccgtcgcc gctggtggcg caggtggcct gaatgtacca gttcctgtgg
      181 cgtagtgagt gcagccccag cttgtctgtg atctcggccg cattcatggc gttggggagg
      241 tcctgcttgt tggcgaacac caggaggaca gcatcccgga gctcgtcctc ggccagcatc
      301 ctcatgagct cctcacgggc ctcgttcaca cgctctctgt cattgctgtc caccacgaag
      361 atcaggcctt gtgtgttctg gaagtagtgg cgccacaggg gccggatctt gtcctggcca
      421 cccacgtccc acacagtgaa gctgatgttc ttgtactcca cggtttccac gttgaagcct
      481 atggtgggaa tggtggtcac gatctcaccc agcttaagct tgtagaggat cgtggtcttc
      541 cctgcagcat ccaggcccac catgaggatg cgcatttctt ttttgccaaa aaggcccttg
      601 aagaggttgg cgaagatgtt ccccatggtt ctatctcctt ctaagcctgc ttttttgtac
      661 aaacttgttt gatcaagctt aactagccag cttgggtctc cctatagtga gtcgtattaa
      721 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
      781 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      841 ttggaaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      901 agacttggaa atccccgtga gtcaaaccgc tatccacgcc catttgatgt actgccaaaa
      961 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tact
//
