LOCUS       sequence-118983-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf3 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF3-GFP
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(642..666)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(698..717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(699..717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(762..861)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(791..811)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt ccttcttgtt tttgagctga ttggccagcc agtccaggcc
      121 ttcgtacagc ccgtccccgc tggtggcaca ggtggcctga atgtaccagt tacggtgacg
      181 aagggaatgc aggcccagct tgtctgtgat ctcagcagcg ttcatagcat taggcagatc
      241 ctgtttgttt gcaaagacaa ggagtacagc atcccggagc tcgtcctccg ccagcattct
      301 catcagctct tcccgggcct catttactcg ctcccgatca ttgctgtcga ccacaaatat
      361 caacccttgg gtgttctgga agtagtgtct ccagaggggt cgaatcttgt cctggccacc
      421 cacatcccac actgtaaagc tgatgttctt atactccact gtctccacat tgaacccaat
      481 ggtagggatg gtggtgacga tctcccccag tttcagcttg tataggatgg tggtctttcc
      541 tgcggcatcc aggcccacca tcaggatgcg catctccttc ttcccaatca ggctcttgag
      601 aaggtttcca aagatattgc ccatggttct atctccttct aagcctgctt ttttgtacaa
      661 acttgtttga tcaagcttaa ctagccagct tgggtctccc tatagtgagt cgtattaatt
      721 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
      781 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      841 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      901 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgt
//
