LOCUS       sequence-118984-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Cronin JC, Wunderlich J, Loftus SK, Prickett TD, Wei X, Ridd K, 
            Vemula S, Burrell AS, Agrawal NS, Lin JC, Banister CE, Buckhaults P,
            Rosenberg SA, Bastian BC, Pavan WJ, Samuels Y
  TITLE     Frequent mutations in the MITF pathway in melanoma.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Aug . 22(4):435-44.
  PUBMED    19422606
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pigment 
            Cell Melanoma Res. 2009 Aug . 22(4):435-44."
COMMENT     Sequence Label: pCMV-Tag4A-MITF-M (wt)
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..50)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     52..68
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     53..69
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttaattaatt aaggtaccgg gccctactta tcgtcgtcat ccttgtaatc ctcgaggtcg
       61 acggtatcga taagcttaca agtgtgctcc gtctcttcca tgctcatact gctcctccgg
      121 ctgcttgttt tggaagctcc gggggacact gaggaaagga gtggatcagt gacaccgacg
      181 ggagaaaggg tgtcgtccat caggatgtct tccagtttgg atcccatttt tgtggggaca
      241 ctataggctt ggttggcctc agtcccagtt ccgaggttgt tgttgaaggt gatggtgcca
      301 tccgtgagat cgagagttgt tgtacaggtt aggtctgcat gatgctgaag gaggtcttgg
      361 ctgcagttct caagaacggg ttcttgcttg atgatccgat tcaccaaatc tggagagcag
      421 agacccgtgg atggaataag ggaaagtcca tgagctcgag cctgcatttc aagttcctgt
      481 attctgagca acaaatgccg gttggcgtgc tccagtttct tctgtcggtt ttcaagttct
      541 tttgcgcgtt gctgttctcg ttgcaacttt cggatatagt ccacggatgc ttttaagatg
      601 gttcccttgt tccagcgcat gtctggatca tttgacttgg gaatcaaagt acctagttct
      661 ttaatgcggt catttatgtt aaatcttctt cttcgttcaa tcaggttgtg attgtccttt
      721 ttctgcctct ctttggccag tgctcttgct tcagactctg tgagctccct ttttatgttg
      781 ggaaggttgg ctggacagga gttgctgatg gtgaggcctg gtgggggcag accttggttt
      841 ccataaagat caatcaagtt tcccgagaca ggcaacgtat ttgccatttg caaagcagga
      901 tccatcaagc ccaagatttc ctcattataa cttgattcta ggc
//
