LOCUS       sequence-118988-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf5 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF5-GFP
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(638..662)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(694..713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(695..713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(758..857)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(787..807)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attgggacaa ctccagtgaa aagttcttct cctttgctag ccattctaga tcgaaccact
       61 ttgtacaaga aagctgggtc gcgctttgac agctcgtggg acagccagtc cagaccatcg
      121 tacagacctg tgccttgggt ggcacaggtg gcctggacat accacgtgcg gctgcgtaag
      181 tgctgtagcc ccagcttgtc agtcagctcg ctcacgggca tggcgttggg catgtcctgc
      241 ttgttggcaa ataccagcag cactgcatcc cgcagctcgt cctcctgcag catcttctgg
      301 agttcatcag cagattcttg gacccgctcc cggtcattac tgtccaccac aaagatgagg
      361 ccctgagtgt tctggaagta gtgccgccac agaggccgaa tcttgtcctg gcctcccacg
      421 tcccagactg tgaaacagat gttcttatat tccactgttt ctacattgaa gcctatggtt
      481 gggatggtgg tgacaatctc ccccaacttc agtttgtaca ggattgtggt cttgccagcc
      541 gcatccaagc caaccatgag aatccgcatc tgcttcttcc cgaagatccg cgaaaagagc
      601 gcggacacgg tgaggcccat ggttctatct ccttctaagc ctgctttttt gtacaaactt
      661 gtttgatcaa gcttaactag ccagcttggg tctccctata gtgagtcgta ttaatttcga
      721 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
      781 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      841 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      901 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtac
//
