LOCUS       sequence-118994-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-q construct contains internal insertions of GFP
            and the EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Hughes TE, Zhang H, Logothetis DE, Berlot CH
  TITLE     Visualization of a functional Galpha q-green fluorescent protein 
            fusion in living cells. Association with the plasma membrane is 
            disrupted by mutational activation and by elimination of 
            palmitoylation sites, but not be activation mediated by receptors or
            AlF4-.
  JOURNAL   J Biol Chem. 2001 Feb 9;276(6):4227-35. Epub 2000 Nov 13.
  PUBMED    11076942
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-02-9"; volume: "276(6)"; pages: "4227-35. Epub 
            2000 Nov 13"
COMMENT     Sequence Label: G protein alpha-q-GFP
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(631..652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattctgcag
      121 atatccatca cactggcggc cgccaccgcg gtgaagctcc gcgccggcgg ggctgcagcg
      181 aggcacttcg gaagaatgac tctggagtcc atcatggcgt gctgcctgag cgaggaggcc
      241 aaggaagccc ggaggatcaa cgacgagatc gagcggcagc tgcgcaggga caagcgcgac
      301 gcccgccggg aactcaagct gctgctgctg gggacagggg agagtggcaa gagcaccttc
      361 atcaagcaga tgaggatcat ccacgggtcg ggctactctg acgaagacaa gcgcggcttc
      421 accaagctgg tgtatcagaa catcttcacg gccatgcagg ccatgatcag agcgatggac
      481 acactcaaga tcccatacaa gtatgaacac aataaggctc atgcacaatt ggttcgagag
      541 gttgatgtgg agaaggtgtc tgctttttct ggtggaggtg gatccatggt gagcaagggc
      601 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      661 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      721 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      781 acctacggcg tgcagtgctt cagccgcta
//
