LOCUS       sequence-118998-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal YFP fragment was fused to Ggamma2. When 
            co-expressed with a carboxyl terminal YFP or CFP fragment fused to a
            Gbeta subunit with which it interacts, a fluorescent signal is 
            produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: YFP(1-158)-gamma-2 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(90..92,93..95,96..554)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(135..156)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(396..415)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctgagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgagctcgga tccgccacca tggtgagcaa gggcgaggag ctgttcaccg
      121 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      181 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      241 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt
      301 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      361 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      421 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      481 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      541 tctatatcat ggccgacaag cagagatcca tggccagcaa caacaccgcc agcatagcac
      601 aagccaggaa actggtagaa cagctgaaga tggaagccaa catcgatagg ataaaggtgt
      661 ccaaggcagc tgcagatttg atggcctact gtgaagcgca tgccaaggaa gatcccctcc
      721 tgacacctgt tccggcttca gaaaacccat ttagggagaa gaagtttttc tgtgctatcc
      781 tttaaagatc tatcacactg gcggccgctc gagcat
//
