LOCUS       sequence-118999-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal YFP fragment was fused to Ggamma2. When 
            co-expressed with a carboxyl terminal YFP or CFP fragment fused to a
            Gbeta subunit with which it interacts, a fluorescent signal is 
            produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: YFP(1-158)-gamma-2 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(241..699,700..702,703..705))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     380..399
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     639..660
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      702..711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(742..761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(743..761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatagatctt taaaggatag cacagaaaaa cttcttctcc ctaaatgggt tttctgaagc
       61 cggaacaggt gtcaggaggg gatcttcctt ggcatgcgct tcacagtagg ccatcaaatc
      121 tgcagctgcc ttggacacct ttatcctatc gatgttggct tccatcttca gctgttctac
      181 cagtttcctg gcttgtgcta tgctggcggt gttgttgctg gccatggatc tctgcttgtc
      241 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
      301 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      361 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
      421 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
      481 gtagcgggcg aagcacatca ggccgtagcc gaaggtggtc acgagggtgg gccagggcac
      541 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      601 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      661 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cggatccgag
      721 ctcggtacca agcttgggtc tccctatagt gagtcgtatt aatttcgata a
//
