LOCUS       sequence-119004-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. 
            coli Expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pSMART-Ala1
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(117..136)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(118..136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtctttcttt tgggtatagc gtcgtggaca gtcattcatc tttctgcccc tccaaaagca
       61 aaaacccgcc gaagcgggtt tttacgtaaa tcaggtgaaa ctgaccgata agccggccct
      121 atagtgagtc gtattagaag agcttactat gcaccagcga cactcgattc gtcttgtaag
      181 gctttttccg ccggtacata ttcgtacccc aaatcacgcg cgaccgcttc atatgtcacg
      241 tgaccgttag cggtgttcag accggcacgt aacgccgtat tgtcggccag cgctttcacg
      301 gcacctttgt tcgcgatttg caacgcatac ggcacggtga catttgtcag tgcgattgta
      361 ctcgtacgtg ggaccgcacc aggcatattc gccaccgcat agtgtacgac accatgtttt
      421 tcatacgtcg gctggtcgtg agtggtaata tggtccaccg tttccacgat gccaccctga
      481 tcaatagcca catcaacaat gactgaaccc ggtttcattt gtttcaccat ttcttccgtc
      541 accaatgtag gtgctttcgc tcccggaatt aacacggcgc agatcaacaa atcagcttcc
      601 gccaccgcat ccgcgatatt gactgggttc gaaatcaggg ttttgatttg gtggccaaag
      661 atatcgtcta attgacgaag gcgatcggca ttacgcgcaa tgattgttac gtcggccccc
      721 aaaccgacgg ccattttggc agcatttgtt cctaccacgc cgccaccaat aatagttact
      781 ttcccacgac tgacgcccgg cactcctgct aagagaatac ccttcccgcc ctttggtttt
      841 tcaagaaact gtgcgccgat ctgcgcagcc atacggcctg ccacttctga catcggggtc
      901 agtaatggca gcgtgcgccc ttcgctgaca gtttcgtatg cgatggccgt gacaccttta
      961 tctttaaggg cttgtgcgag ctccggttca gctgccagat gcagatatgt aaacagcacc
     1021 agacctttac gaaaatagac atattcttcc gggagcggtt ctttcacttt catgaccatc
     1081 tctgcatccc agacctgttt
//
