LOCUS       sequence-119009-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + 
            PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2085
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    275..293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(314..332)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        400..504
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(723..742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctcgcgcca acaattccac acatagtttt tgattccgat ttatatcgtt gtcggtcaac
       61 ctgtatgccc gttttttcgg gcgccccgag ccttgtagag agtggggtta tttttatagt
      121 tactgcttgt gggagggaga ttatgttatc tcctcatccc atgtgaccgg gttagccggc
      181 cagaatcact ttttctttac cggacgacgc cagccttctt tctgagtctg cggcacacgg
      241 tcgacagtat ctctatcact gatagggatg tcaatctcta tcactgatag ggactcgagg
      301 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      361 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcg
//
