LOCUS       sequence-119036-        3362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the LexA Binding Domain fused to SRDX Transcriptional 
            Repressor as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pLexA::SRDX (GB0732)
FEATURES             Location/Qualifiers
     source          1..3362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..257
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     promoter        complement(413..431)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(414..431)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(449..465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(449..465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(462..484)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    473..489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(497..503,504..521,522..527))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    542..563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(680..697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(851..1439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(931..950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1610..2401,2402..2470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGPRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2233..2252
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2471..2575)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2653..3108)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2790..2811)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3002..3021
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3234..3256
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3248..3265
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3249..3265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3272..3291
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3272..3290
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3321..3330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3325..3334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca
       61 tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt
      121 ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt attgaaattg
      181 tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg
      241 taggtcgtgt ggctgccggt gaaccggagc cacttgatct tgatcttgaa cttagacttg
      301 gttttgctta ggcttcgaga ccactcatcg cccatcacta gtgaattcgc ggccgcctgc
      361 aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt
      421 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      601 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1561 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1621 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1681 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1741 tgataccgcg aggcccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1801 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1861 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1921 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1981 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2041 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2101 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2161 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2221 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2281 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2341 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2401 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2461 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2521 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2581 ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2641 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2761 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2821 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgctggc ccactacgtg
     2881 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2941 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3001 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3061 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     3121 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     3181 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     3241 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg
     3301 cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgatgg gcgatgagtg
     3361 gt
//
