LOCUS       sequence-119037-        3326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Gal4 Binding Domain fused to the BDR Transcriptional 
            Repressor as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pGal4BD::BDR (GB0734)
FEATURES             Location/Qualifiers
     source          1..3326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(377..395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(378..395)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(413..429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(413..429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(426..448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    437..453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(461..467,468..485,486..491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    506..527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(644..661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(815..1403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(895..914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1574..2365,2366..2434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGPRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2197..2216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2435..2539)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2617..3072)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2754..2775)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2966..2985
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3198..3220
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3212..3229
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3213..3229
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3236..3255
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3236..3254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3285..3294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3289..3298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgaatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
       61 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
      121 gctactctcc caaaaccaaa agatctccgc tgactagggc acatctgaca gaagtggaat
      181 caaggctaga aagactggaa cagctatttc tactgatttt tcctaggggt aattctaaga
      241 ctcttagact ttttggtgtt aatatggaat gttgagcttc gagaccactc atcgcccatc
      301 actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga
      361 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
      421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1681 taccatctgg ccccagtgct gcaatgatac cgcgaggccc acgctcaccg gctccagatt
     1741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2461 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2521 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata
     2581 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
     2641 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2701 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2761 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2821 atcagggcgc tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2881 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2941 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3001 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3061 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3121 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3181 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     3241 cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc atggcggccg
     3301 cgggaattcg atgggcgatg agtggt
//
