LOCUS       sequence-119063-        4205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the bar gene for resistance to basta fused to the A. 
            tumefaciens Nopaline synthase promoter and terminator. Cloned as a 
            level 0 GB part ready to be transferred to a GB level1 plant 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTNos:BASTA:PNos (GB0023)
FEATURES             Location/Qualifiers
     source          1..4205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..280)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(300..851)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(893..1072)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1256..1274)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1257..1274)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1292..1308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1292..1308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1305..1327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1316..1332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1340..1346,1347..1364,1365..1370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1385..1406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1523..1540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1694..2282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1774..1793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2453..3244,3245..3313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3076..3095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3314..3418)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3496..3951)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3633..3654)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3845..3864
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4077..4099
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4091..4108
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4092..4108
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4115..4134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4115..4133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4164..4173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4168..4177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggagat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata
       61 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
      121 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      181 catgcttaac gtaattcaac agaaattata tgataatcat cccaagaccg gcaacaggat
      241 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat
      301 cagatttcgg tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc
      361 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg
      421 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca
      481 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg
      541 gagcccagtc ccgtccgctg gtggcggggg gacacgtaca cggtcgactc ggccgtccag
      601 tcgtaggcgt tgcgtgcctt ccagggaccc gcgtaggcta tgccggcgac ctcgccgtcc
      661 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc
      721 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg
      781 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt
      841 tctgggctca tggtagatcc cctcgatcga gttgagagtg aatatgagac tctaattgga
      901 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
      961 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     1021 tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt
     1081 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     1141 tctcatgtat gatacgctcg agaccactca tcgcccatca ctagtgaatt cgcggccgcc
     1201 tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat
     1261 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2581 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3421 catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3481 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3541 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3601 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3661 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3721 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3781 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3841 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3901 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     3961 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     4021 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     4081 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     4141 gggcccgacg tcgcatgctc ccggccgcca tggcggccgc gggaattcga tgggcgatga
     4201 gtggt
//
