LOCUS       sequence-119099-        1591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: MB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_1_MB1
FEATURES             Location/Qualifiers
     source          1..1591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(32..620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(112..131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        659..730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(731..799,800..1591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(949..968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tctggtgggt ctctgtcccg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
       61 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      601 gcagaaaaaa aggatctcaa gaagatcctt tgaggctcgg gagacctatc gctaaataca
      661 ttcaaatatc tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1021 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1081 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1141 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1201 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1261 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1321 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1381 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1441 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1501 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1561 gataggtgcc tccctgatta agcattggta a
//
