LOCUS       sequence-119101-        3987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: Kan.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_3_Kan
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             145..960
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             145..960
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(218..237)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(1061..1080)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1062..1080)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1386..1404)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1472..1576
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1577..1645,1646..2437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1795..1814)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2608..3196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3097..3116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3350..3367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3487..3508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3523..3528,3529..3546,3547..3553)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 tctggtgggt ctctgtccgt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc
       61 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      121 ataaacagta atacaagtgg tgttatgagc catattcaac gggaaacgtc ttgctccagg
      181 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      241 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
      301 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
      361 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
      421 gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa
      481 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
      541 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
      601 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
      661 tggcctgttg aacaagtctg gaaggaaatg cataagcttt tgccattctc accggattca
      721 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
      781 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
      841 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
      901 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
      961 tcagaattgg ttaattggtt gtaacactgg cagagggctc gggagaccta tcgatcttgc
     1021 tgaaaaactc gagccatccg gaagatctgg cggccgctct ccctatagtg agtcgtatta
     1081 cgccggatgg atatggtgtt caggcacaag tgttaaagca gttgatttta ttcactatga
     1141 tgaaaaaaac aatgaatgga acctgctcca agttaaaaat agagataata ccgaaaactc
     1201 atcgagtagt aagattagag ataatacaac aataaaaaaa tggtttagaa cttactcaca
     1261 gcgtgatgct actaattggg acaattttcc agatgaagta tcatctaaga atttaaatga
     1321 agaagacttc agagcttttg ttaaaaatta tttggcaaaa ataatataat tcggctgcag
     1381 gggcggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1441 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1501 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1561 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1621 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1681 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1741 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1801 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1861 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1921 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1981 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2041 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2101 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2161 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2221 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2281 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2341 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2401 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2461 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2521 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2581 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2641 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2701 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2761 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2821 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2881 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2941 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3001 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3061 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3121 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3181 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3241 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3301 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3361 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3421 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcaattggca gtgagcgcaa
     3481 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3541 ggctcgtata atgtgtggaa ttatgagcgg ataataattt cacacaggag gtttaaactt
     3601 taaacatgtc aaaagagacg tcttttgtta agaatgctga ggaacttgca aagcaaaaaa
     3661 tggatgctat taaccctgaa ctttcttcaa aatttaaatt tttaataaaa ttcctgtctc
     3721 agtttcctga agcttgctct aaacctcgtt caaaaaaaat gcagaataaa gttggtcaag
     3781 aggaacatat tgaatattta gctcgtagtt ttcatgagag tcgattgcca agaaaaccca
     3841 cgccacctac aacggttcct gatgaggtgg ttagcatagt tcttaatata agttttaata
     3901 tacagcctga aaatcttgag agaataaaag aagaacatcg attttccatg gcagctgaga
     3961 atattgtagg agatcttcta gaaagat
//
