LOCUS       sequence-119102-        3970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: Cm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_4_Cm
FEATURES             Location/Qualifiers
     source          1..3970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..167
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             168..827
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(234..253)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1431..1437,1438..1455,1456..1461))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1476..1497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1617..1634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1788..2376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1868..1887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2547..3338,3339..3407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3170..3189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3408..3512)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3580..3598
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3904..3923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3904..3922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggtgggt ctctgtcctt gataccggga agccctgggc caacttttgg cgaaaatgag
       61 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      121 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      181 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      241 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      301 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      361 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
      421 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
      481 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      541 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
      601 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
      661 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
      721 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
      781 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
      841 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
      901 atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat
      961 agccgcttat gtctattggg ctcgggagac ctatcgatct ttctagaaga tctcctacaa
     1021 tattctcagc tgccatggaa aatcgatgtt cttcttttat tctctcaaga ttttcaggct
     1081 gtatattaaa acttatatta agaactatgc taaccacctc atcaggaacc gttgtaggtg
     1141 gcgtgggttt tcttggcaat cgactctcat gaaaactacg agctaaatat tcaatatgtt
     1201 cctcttgacc aactttattc tgcatttttt ttgaacgagg tttagagcaa gcttcaggaa
     1261 actgagacag gaattttatt aaaaatttaa attttgaaga aagttcaggg ttaatagcat
     1321 ccattttttg ctttgcaagt tcctcagcat tcttaacaaa agacgtctct tttgacatgt
     1381 ttaaagttta aacctcctgt gtgaaattat tatccgctca taattccaca cattatacga
     1441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1501 gcgttgcgct cactgccaat tgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1561 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2221 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2461 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3121 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3181 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3241 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3301 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3361 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3421 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3481 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3541 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccg
     3601 cccctgcagc cgaattatat tatttttgcc aaataatttt taacaaaagc tctgaagtct
     3661 tcttcattta aattcttaga tgatacttca tctggaaaat tgtcccaatt agtagcatca
     3721 cgctgtgagt aagttctaaa ccattttttt attgttgtat tatctctaat cttactactc
     3781 gatgagtttt cggtattatc tctattttta acttggagca ggttccattc attgtttttt
     3841 tcatcatagt gaataaaatc aactgcttta acacttgtgc ctgaacacca tatccatccg
     3901 gcgtaatacg actcactata gggagagcgg ccgccagatc ttccggatgg ctcgagtttt
     3961 tcagcaagat
//
