LOCUS       sequence-119103-        3951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: Amp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_5_Amp
FEATURES             Location/Qualifiers
     source          1..3951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(19..810,811..879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(880..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1025..1044)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1026..1044)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1350..1368)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1436..1540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1541..1609,1610..2401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2572..3160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3061..3080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3314..3331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3451..3472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3487..3492,3493..3510,3511..3517)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 tctggtgggt ctctgtcctt accaatgctt aatcagtgag gcacctatct cagcgatctg
       61 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      121 gggcttacca tctggcccca gtgctgcaat gataccgcga gatccacgct caccggctcc
      181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
      721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
      781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
      841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttaggg
      961 ctcgggagac ctatcgtatc ttgctgaaaa actcgagcca tccggaagat ctggcggccg
     1021 ctctccctat agtgagtcgt attacgccgg atggatatgg tgttcaggca caagtgttaa
     1081 agcagttgat tttattcact atgatgaaaa aaacaatgaa tggaacctgc tccaagttaa
     1141 aaatagagat aataccgaaa actcatcgag tagtaagatt agagataata caacaataaa
     1201 aaaatggttt agaacttact cacagcgtga tgctactaat tgggacaatt ttccagatga
     1261 agtatcatct aagaatttaa atgaagaaga cttcagagct tttgttaaaa attatttggc
     1321 aaaaataata taattcggct gcaggggcgg cctcgtgata cgcctatttt tataggttaa
     1381 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2701 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3181 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3241 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3361 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     3421 gaaagcaatt ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3481 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggaattatga gcggataata
     3541 atttcacaca ggaggtttaa actttaaaca tgtcaaaaga gacgtctttt gttaagaatg
     3601 ctgaggaact tgcaaagcaa aaaatggatg ctattaaccc tgaactttct tcaaaattta
     3661 aatttttaat aaaattcctg tctcagtttc ctgaagcttg ctctaaacct cgttcaaaaa
     3721 aaatgcagaa taaagttggt caagaggaac atattgaata tttagctcgt agttttcatg
     3781 agagtcgatt gccaagaaaa cccacgccac ctacaacggt tcctgatgag gtggttagca
     3841 tagttcttaa tataagtttt aatatacagc ctgaaaatct tgagagaata aaagaagaac
     3901 atcgattttc catggcagct gagaatattg taggagatct tctagaaaga t
//
