LOCUS       sequence-119105-        3922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: RFP expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_7_J23101-RBS34-mCherry-B0015
FEATURES             Location/Qualifiers
     source          1..3922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..53
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     RBS             62..73
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             80..790
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(225..243)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     493..512
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      810..881
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      897..924
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(join(1383..1389,1390..1407,1408..1413))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1428..1449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1569..1586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1740..2328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1820..1839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2499..3290,3291..3359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3122..3141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3360..3464)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3532..3550
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3856..3875
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3856..3874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggtgggt ctctgtcctt tacagctagc tcagtcctag gtattatgct agctactaga
       61 gaaagaggag aaatactaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      121 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      181 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      241 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      301 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      361 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      421 ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      481 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      541 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
      601 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      661 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
      721 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
      781 gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata ggctcgggag acctatcgat ctttctagaa
      961 gatctcctac aatattctca gctgccatgg aaaatcgatg ttcttctttt attctctcaa
     1021 gattttcagg ctgtatatta aaacttatat taagaactat gctaaccacc tcatcaggaa
     1081 ccgttgtagg tggcgtgggt tttcttggca atcgactctc atgaaaacta cgagctaaat
     1141 attcaatatg ttcctcttga ccaactttat tctgcatttt ttttgaacga ggtttagagc
     1201 aagcttcagg aaactgagac aggaatttta ttaaaaattt aaattttgaa gaaagttcag
     1261 ggttaatagc atccattttt tgctttgcaa gttcctcagc attcttaaca aaagacgtct
     1321 cttttgacat gtttaaagtt taaacctcct gtgtgaaatt attatccgct cataattcca
     1381 cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1441 ctcacattaa ttgcgttgcg ctcactgcca attgctttcc agtcgggaaa cctgtcgtgc
     1501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2161 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3481 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3541 atcacgaggc cgcccctgca gccgaattat attatttttg ccaaataatt tttaacaaaa
     3601 gctctgaagt cttcttcatt taaattctta gatgatactt catctggaaa attgtcccaa
     3661 ttagtagcat cacgctgtga gtaagttcta aaccattttt ttattgttgt attatctcta
     3721 atcttactac tcgatgagtt ttcggtatta tctctatttt taacttggag caggttccat
     3781 tcattgtttt tttcatcata gtgaataaaa tcaactgctt taacacttgt gcctgaacac
     3841 catatccatc cggcgtaata cgactcacta tagggagagc ggccgccaga tcttccggat
     3901 ggctcgagtt tttcagcaag at
//
