LOCUS       sequence-119108-        3935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: GFP expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_10_J23101-RBS32-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..3935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..53
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             81..794
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      823..894
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      910..937
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(join(1396..1402,1403..1420,1421..1426))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1441..1462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1582..1599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1753..2341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1833..1852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2512..3303,3304..3372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3135..3154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3373..3477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3545..3563
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3869..3888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3869..3887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggtgggt ctctgtcctt tacagctagc tcagtcctag gtattatgct agctactaga
       61 gtcacacagg aaagtactag atgcgtaaag gcgaagaact gttcacgggc gtagttccga
      121 ttctggtcga gctggacggc gatgtgaacg gtcataagtt tagcgttcgc ggtgaaggtg
      181 agggcgacgc gaccaacggc aaactgaccc tgaagttcat ctgcaccacc ggtaaactgc
      241 cggtgccttg gccgaccttg gtgacgacgt tgacgtatgg cgtgcagtgt tttgcgcgtt
      301 atccggacca catgaaacaa cacgatttct tcaaatctgc gatgccggag ggttacgtcc
      361 aggagcgtac catttccttc aaggatgatg gcacttacaa aactcgcgca gaggttaagt
      421 ttgaaggtga cacgctggtc aatcgtatcg aattgaaggg tatcgacttt aaagaggatg
      481 gtaacattct gggccataaa ctggagtata acttcaacag ccataatgtt tacattacgg
      541 cagacaagca aaagaacggc atcaaggcca atttcaagat tcgccacaat gttgaggacg
      601 gtagcgtcca actggccgac cattaccagc agaacacccc aattggtgac ggtccggttt
      661 tgctgccgga taatcactat ctgagcaccc aaagcgtgct gagcaaagat ccgaacgaaa
      721 aacgtgatca catggtcctg ctggaatttg tgaccgctgc gggcatcacc cacggtatgg
      781 acgagctgta taagcgtccg taataatact agagccaggc atcaaataaa acgaaaggct
      841 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      901 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggctcgg gagacctatc
      961 gatctttcta gaagatctcc tacaatattc tcagctgcca tggaaaatcg atgttcttct
     1021 tttattctct caagattttc aggctgtata ttaaaactta tattaagaac tatgctaacc
     1081 acctcatcag gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact ctcatgaaaa
     1141 ctacgagcta aatattcaat atgttcctct tgaccaactt tattctgcat tttttttgaa
     1201 cgaggtttag agcaagcttc aggaaactga gacaggaatt ttattaaaaa tttaaatttt
     1261 gaagaaagtt cagggttaat agcatccatt ttttgctttg caagttcctc agcattctta
     1321 acaaaagacg tctcttttga catgtttaaa gtttaaacct cctgtgtgaa attattatcc
     1381 gctcataatt ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1441 atgagtgagc taactcacat taattgcgtt gcgctcactg ccaattgctt tccagtcggg
     1501 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2161 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2641 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3481 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3541 taaaaatagg cgtatcacga ggccgcccct gcagccgaat tatattattt ttgccaaata
     3601 atttttaaca aaagctctga agtcttcttc atttaaattc ttagatgata cttcatctgg
     3661 aaaattgtcc caattagtag catcacgctg tgagtaagtt ctaaaccatt tttttattgt
     3721 tgtattatct ctaatcttac tactcgatga gttttcggta ttatctctat ttttaacttg
     3781 gagcaggttc cattcattgt ttttttcatc atagtgaata aaatcaactg ctttaacact
     3841 tgtgcctgaa caccatatcc atccggcgta atacgactca ctatagggag agcggccgcc
     3901 agatcttccg gatggctcga gtttttcagc aagat
//
