LOCUS       sequence-119109-        3891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: RBS-GFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_11_RBS34-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..3891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             19..30
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             37..750
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      779..850
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      866..893
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(join(1353..1359,1360..1377,1378..1383))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1398..1419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1539..1556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1710..2298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1790..1809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2469..3260,3261..3329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3092..3111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3330..3434)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3502..3520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3826..3845
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3826..3844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggtgggt ctctgtccaa agaggagaaa tactagatgc gtaaaggcga agaactgttc
       61 acgggcgtag ttccgattct ggtcgagctg gacggcgatg tgaacggtca taagtttagc
      121 gttcgcggtg aaggtgaggg cgacgcgacc aacggcaaac tgaccctgaa gttcatctgc
      181 accaccggta aactgccggt gccttggccg accttggtga cgacgttgac gtatggcgtg
      241 cagtgttttg cgcgttatcc ggaccacatg aaacaacacg atttcttcaa atctgcgatg
      301 ccggagggtt acgtccagga gcgtaccatt tccttcaagg atgatggcac ttacaaaact
      361 cgcgcagagg ttaagtttga aggtgacacg ctggtcaatc gtatcgaatt gaagggtatc
      421 gactttaaag aggatggtaa cattctgggc cataaactgg agtataactt caacagccat
      481 aatgtttaca ttacggcaga caagcaaaag aacggcatca aggccaattt caagattcgc
      541 cacaatgttg aggacggtag cgtccaactg gccgaccatt accagcagaa caccccaatt
      601 ggtgacggtc cggttttgct gccggataat cactatctga gcacccaaag cgtgctgagc
      661 aaagatccga acgaaaaacg tgatcacatg gtcctgctgg aatttgtgac cgctgcgggc
      721 atcacccacg gtatggacga gctgtataag cgtccgtaat aatactagag ccaggcatca
      781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag
      901 gctcgggaga cctatcgtat ctttctagaa gatctcctac aatattctca gctgccatgg
      961 aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta aaacttatat
     1021 taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt tttcttggca
     1081 atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga ccaactttat
     1141 tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaactgagac aggaatttta
     1201 ttaaaaattt aaattttgaa gaaagttcag ggttaatagc atccattttt tgctttgcaa
     1261 gttcctcagc attcttaaca aaagacgtct cttttgacat gtttaaagtt taaacctcct
     1321 gtgtgaaatt attatccgct cataattcca cacattatac gagccggaag cataaagtgt
     1381 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgcca
     1441 attgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1501 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1561 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2581 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3421 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3481 atcatgacat taacctataa aaataggcgt atcacgaggc cgcccctgca gccgaattat
     3541 attatttttg ccaaataatt tttaacaaaa gctctgaagt cttcttcatt taaattctta
     3601 gatgatactt catctggaaa attgtcccaa ttagtagcat cacgctgtga gtaagttcta
     3661 aaccattttt ttattgttgt attatctcta atcttactac tcgatgagtt ttcggtatta
     3721 tctctatttt taacttggag caggttccat tcattgtttt tttcatcata gtgaataaaa
     3781 tcaactgctt taacacttgt gcctgaacac catatccatc cggcgtaata cgactcacta
     3841 tagggagagc ggccgccaga tcttccggat ggctcgagtt tttcagcaag a
//
