LOCUS       sequence-119110-        3873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: GFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_12_ATG-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..3873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..732
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      761..832
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      848..875
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(join(1335..1341,1342..1359,1360..1365))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1380..1401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1521..1538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1692..2280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1772..1791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2451..3242,3243..3311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3074..3093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3312..3416)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3484..3502
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3808..3827
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3808..3826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggtgggt ctctgtccat gcgtaaaggc gaagaactgt tcacgggcgt agttccgatt
       61 ctggtcgagc tggacggcga tgtgaacggt cataagttta gcgttcgcgg tgaaggtgag
      121 ggcgacgcga ccaacggcaa actgaccctg aagttcatct gcaccaccgg taaactgccg
      181 gtgccttggc cgaccttggt gacgacgttg acgtatggcg tgcagtgttt tgcgcgttat
      241 ccggaccaca tgaaacaaca cgatttcttc aaatctgcga tgccggaggg ttacgtccag
      301 gagcgtacca tttccttcaa ggatgatggc acttacaaaa ctcgcgcaga ggttaagttt
      361 gaaggtgaca cgctggtcaa tcgtatcgaa ttgaagggta tcgactttaa agaggatggt
      421 aacattctgg gccataaact ggagtataac ttcaacagcc ataatgttta cattacggca
      481 gacaagcaaa agaacggcat caaggccaat ttcaagattc gccacaatgt tgaggacggt
      541 agcgtccaac tggccgacca ttaccagcag aacaccccaa ttggtgacgg tccggttttg
      601 ctgccggata atcactatct gagcacccaa agcgtgctga gcaaagatcc gaacgaaaaa
      661 cgtgatcaca tggtcctgct ggaatttgtg accgctgcgg gcatcaccca cggtatggac
      721 gagctgtata agcgtccgta ataatactag agccaggcat caaataaaac gaaaggctca
      781 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      841 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat aggctcggga gacctatcgt
      901 atctttctag aagatctcct acaatattct cagctgccat ggaaaatcga tgttcttctt
      961 ttattctctc aagattttca ggctgtatat taaaacttat attaagaact atgctaacca
     1021 cctcatcagg aaccgttgta ggtggcgtgg gttttcttgg caatcgactc tcatgaaaac
     1081 tacgagctaa atattcaata tgttcctctt gaccaacttt attctgcatt ttttttgaac
     1141 gaggtttaga gcaagcttca ggaaactgag acaggaattt tattaaaaat ttaaattttg
     1201 aagaaagttc agggttaata gcatccattt tttgctttgc aagttcctca gcattcttaa
     1261 caaaagacgt ctcttttgac atgtttaaag tttaaacctc ctgtgtgaaa ttattatccg
     1321 ctcataattc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1381 tgagtgagct aactcacatt aattgcgttg cgctcactgc caattgcttt ccagtcggga
     1441 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1501 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1561 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1621 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1681 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1741 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1801 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1861 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1921 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1981 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2041 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2101 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2161 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2221 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2281 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2341 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2401 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2461 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2521 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2581 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2641 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2701 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2761 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2821 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2881 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2941 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3001 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3061 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3121 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3181 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3241 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3301 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3361 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3421 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3481 aaaaataggc gtatcacgag gccgcccctg cagccgaatt atattatttt tgccaaataa
     3541 tttttaacaa aagctctgaa gtcttcttca tttaaattct tagatgatac ttcatctgga
     3601 aaattgtccc aattagtagc atcacgctgt gagtaagttc taaaccattt ttttattgtt
     3661 gtattatctc taatcttact actcgatgag ttttcggtat tatctctatt tttaacttgg
     3721 agcaggttcc attcattgtt tttttcatca tagtgaataa aatcaactgc tttaacactt
     3781 gtgcctgaac accatatcca tccggcgtaa tacgactcac tatagggaga gcggccgcca
     3841 gatcttccgg atggctcgag tttttcagca aga
//
