LOCUS       sequence-119112-        3730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  storage vector for BASIC part: GFP(ORF) -nostop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_13_ATG-GFP-nostop
FEATURES             Location/Qualifiers
     source          1..3730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..732
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(join(1192..1198,1199..1216,1217..1222))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1237..1258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1378..1395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1549..2137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1629..1648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2308..3099,3100..3168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2931..2950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3169..3273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3341..3359
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3665..3684
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3665..3683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggtgggt ctctgtccat gcgtaaaggc gaagaactgt tcacgggcgt agttccgatt
       61 ctggtcgagc tggacggcga tgtgaacggt cataagttta gcgttcgcgg tgaaggtgag
      121 ggcgacgcga ccaacggcaa actgaccctg aagttcatct gcaccaccgg taaactgccg
      181 gtgccttggc cgaccttggt gacgacgttg acgtatggcg tgcagtgttt tgcgcgttat
      241 ccggaccaca tgaaacaaca cgatttcttc aaatctgcga tgccggaggg ttacgtccag
      301 gagcgtacca tttccttcaa ggatgatggc acttacaaaa ctcgcgcaga ggttaagttt
      361 gaaggtgaca cgctggtcaa tcgtatcgaa ttgaagggta tcgactttaa agaggatggt
      421 aacattctgg gccataaact ggagtataac ttcaacagcc ataatgttta cattacggca
      481 gacaagcaaa agaacggcat caaggccaat ttcaagattc gccacaatgt tgaggacggt
      541 agcgtccaac tggccgacca ttaccagcag aacaccccaa ttggtgacgg tccggttttg
      601 ctgccggata atcactatct gagcacccaa agcgtgctga gcaaagatcc gaacgaaaaa
      661 cgtgatcaca tggtcctgct ggaatttgtg accgctgcgg gcatcaccca cggtatggac
      721 gagctgtata agcgtccggg ctcgggagac ctatcgtatc tttctagaag atctcctaca
      781 atattctcag ctgccatgga aaatcgatgt tcttctttta ttctctcaag attttcaggc
      841 tgtatattaa aacttatatt aagaactatg ctaaccacct catcaggaac cgttgtaggt
      901 ggcgtgggtt ttcttggcaa tcgactctca tgaaaactac gagctaaata ttcaatatgt
      961 tcctcttgac caactttatt ctgcattttt tttgaacgag gtttagagca agcttcagga
     1021 aactgagaca ggaattttat taaaaattta aattttgaag aaagttcagg gttaatagca
     1081 tccatttttt gctttgcaag ttcctcagca ttcttaacaa aagacgtctc ttttgacatg
     1141 tttaaagttt aaacctcctg tgtgaaatta ttatccgctc ataattccac acattatacg
     1201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1261 tgcgttgcgc tcactgccaa ttgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1321 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1981 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3301 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3361 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
     3421 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
     3481 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
     3541 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
     3601 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
     3661 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
     3721 ttcagcaaga
//
