LOCUS       sequence-119115-         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector: p53 shRNA under control of mouse U6 
            promoter, EGFP-expression driven by CAG promoter to monitor 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Chih B, Engelman H, Scheiffele P
  TITLE     Control of excitatory and inhibitory synapse formation by 
            neuroligins.
  JOURNAL   Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27.
  PUBMED    15681343
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-02-25"; volume: "307(5713)"; pages: "1324-8. Epub 2005 
            Jan 27"
COMMENT     Sequence Label: pLenLox_U6 p53
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     144..164
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     281..297
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     282..298
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(340..373)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
       61 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa gagatacaaa
      121 tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact gtaaagtaat
      181 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttgtac atgtgtaata
      241 gctccttcaa gagaggagct attacacatg tacttttttc tcgaggtcga cggtatcgat
      301 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
      361 tatacgaagt tatattaagg gttccaagct taagcggccg cgtggataac cgtattaccg
      421 ctcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
//
