LOCUS       sequence-119117-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector: Neuroligin 1 shRNA under control of 
            mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Chih B, Engelman H, Scheiffele P
  TITLE     Control of excitatory and inhibitory synapse formation by 
            neuroligins.
  JOURNAL   Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27.
  PUBMED    15681343
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-02-25"; volume: "307(5713)"; pages: "1324-8. Epub 2005 
            Jan 27"
COMMENT     Sequence Label: pLenLox_U6 Nlgn1
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..160
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     277..293
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     278..294
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(336..369)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
       61 ttttaatact agctacattt tacatgatag gcttggattt ctataagaga tacaaatact
      121 aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
      181 tgttttgaga ctataaatat cccttggaga aaagccttgt ttggaaggta ctggaaatct
      241 attcaagaga tagatttcca gtaccttcct tttttctcga ggtcgacggt atcgataagc
      301 tcgcttcacg agattccagc aggtcgaggg acctaataac ttcgtatagc atacattata
      361 cgaagttata ttaagggttc caagcttaag cggccgcgtg gataaccgta ttaccgccat
      421 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      481 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      541 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      601 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      661 caagtgtatc atatgccaag tac
//
