LOCUS       sequence-119121-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for human KCC2 under CAG promoter. 
            Coexpression of TdTomato with IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Bortone D, Polleux F
  TITLE     KCC2 expression promotes the termination of cortical interneuron 
            migration in a voltage-sensitive calcium-dependent manner.
  JOURNAL   Neuron. 2009 Apr 16;62(1):53-71. doi: 10.1016/j.neuron.2009.01.034.
  PUBMED    19376067
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.01"; journalName: "Neuron"; date: 
            "2009-04-16- 16"; volume: "62"; issue: "1"; pages: "53-71"
COMMENT     Sequence Label: pCITF-KCC2
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(97..115)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     365..384
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     559..582
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             666..689
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
       61 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      121 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
      181 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
      241 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
      301 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
      361 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
      421 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
      481 gctacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
      541 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      601 aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt
      661 acaaggacta taaggacgat gatgacaagt agcggccgca cgcgtcaagg gcgaattggc
      721 cgcgactcta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      781 aa
//
