LOCUS       sequence-119123-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2 with H134R mutation fused to EYFP 
            driven by human Synapsin I promoter for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-hSyn-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..301)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(92..113)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     441..460
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcttac ttgtacagct
       61 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      121 gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca
      181 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      241 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
      301 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      361 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      421 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      481 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      541 ggtagcgggc gaagcactgc aggccgtagc cgaaggtggt cacgagggtg ggccagggca
      601 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
      661 cctcgccctc gccggacacg ct
//
