LOCUS       sequence-119135-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APEX2 fused to the N-terminus of human beta actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: APEX2 - Actin in pEGFP
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             64..87
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atggactaca aggatgacga cgataagggg aaatcatacc caacagtgtc cgcagactac
      121 caggatgccg tggagaaagc caagaagaag ctgagagggt ttatcgcaga aaagaggtgc
      181 gcacctctga tgctgagact ggctttccac agcgcaggca cctttgacaa gggaaccaaa
      241 acaggcggac cctttggaac aatcaagcac cctgctgaac tggcacatag tgccaacaat
      301 gggctggaca tcgccgtgcg gctgctggaa cctctgaaag cagagttccc aattctgtcc
      361 tacgccgatt tttatcagct ggcaggagtg gtcgctgtgg aggtcactgg gggccccaag
      421 gtgcctttcc acccaggacg ggaggacaaa ccagaaccac ctccagaggg gcgcctgcca
      481 gatccgacaa agggctccga ccatctgcga gatgtgtttg ggaaagctat gggcctgact
      541 gaccaggata tcgtcgcact gtctggaggg cacaccattg gcgccgctca taaggaaagg
      601 tcaggcttcg agggaccctg gacaagcaac cccctgattt tcgacaattc ttactttact
      661 gaactgctga gtggagagaa ggaagggctg ctgcagctgc ccagcgacaa agccctgctg
      721 tccgatcccg tgttcagacc tctggtcgat aagtatgcag ccgacgagga tgcttttttc
      781 gcagattacg cagaagcaca tcagaagctg tcagaactgg gatttgccga cgccaagggc
      841 tcgggctcga cctcgggctc gggctccgga ctcagatctc gagctatgga tgatgatatc
      901 gccgcgctcg tcgtcgacaa cggctccggc atgtgcaagg ccggcttcgc gggcgacgat
      961 gccccccggg ccgtcttccc ctccatcgtg gggcgcccca ggcaccaggg cgtgatggtg
     1021 ggcatgggtc agaaggattc ctatgtgggc gacgaggccc agagcaagag aggcatcctc
     1081 acc
//
