LOCUS       sequence-119139-        4772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone pComb3X containing PCR template for human antibody lambda 
            light chain constant region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd
  TITLE     Methods for the generation of chicken monoclonal antibody fragments 
            by phage display.
  JOURNAL   J Immunol Methods. 2000 Aug 28;242(1-2):159-81.
  PUBMED    10986398
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol 
            Methods."; date: "2000-08-28"; volume: "242(1-2)"; pages: "159-81"
COMMENT     Sequence Label: pComb3XLambda
FEATURES             Location/Qualifiers
     source          1..4772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    109..130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(145..150,151..168,169..175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     222..284
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     sig_peptide     963..1028
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             1743..1760
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1767..1793
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      complement(2387..2842)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2524..2545)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2736..2755
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2869..2973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2974..3042,3043..3834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3192..3211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4005..4593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4494..4513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4747..4764
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
       61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      181 aattgtgagc ggataacaat tgaattcagg aggaatttaa aatgaaaaag acagctatcg
      241 cgattgcagt ggcactggct ggtttcgcta ccgtggccca ggcggccgag ctcgtgctga
      301 ctaacgccct cagtgtctgg ggccccaggg cagagggtca ccatctcctg cactgggagc
      361 agctccaacc tcggggcagc ttatgatgta cactggtacc accaggttcc aggaacggcc
      421 cccaaactcc tcctctatga taacatcaag cggccctcag gggtccctga ccgattctct
      481 ggctccaagt ctggcacctc agcctccctg gccatcactg ggctccaggc tgaggacgag
      541 gctgattatt actgcaacag agtaacagct ggcctcacac gttcggcgga gggaccaagc
      601 tgaccgtcct aagtcagccc aaggctgccc cctcggtcac tctgttcccg ccctcctctg
      661 aggagcttca agccaacaag gccacactgg tgtgtctcat aagtgacttc tacccgggag
      721 ccgtgacagt ggcctggaag gcagatggca gccccgtcaa ggcgggagtg gagaccacca
      781 caccctccaa acaaagcaac aacaagtacg cggccagcag ctatctgagc ctgacgcctg
      841 agcagtggaa gtcccacaga agctacagct gccaggtcac gcatgaaggg agcaccgtgg
      901 agaagacagt ggcccctaca gaatgttcat aattctagat aattaattag gaggaattta
      961 aaatgaaata cctattgcct acggcagccg ctggattgtt attactcgct gcccaaccag
     1021 ccatggccga ggtgcagctg ctcgagcagt ctggggctga ggtgaagaag cctgggtcct
     1081 cggtgaaggt ctcctgcagg gcttctggag gcaccttcaa caattatgcc atcagctggg
     1141 tgcgacaggc ccctggacaa gggcttgagt ggatgggagg gatcttccct ttccgtaata
     1201 cagcaaagta cgcacaacac ttccagggca gagtcaccat taccgcggac gaatccacgg
     1261 gcacagccta catggagctg agcagcctga gatctgagga cacggccata tattattgtg
     1321 cgagagggga tacgattttt ggagtgacca tgggatacta cgctatggac gtctggggcc
     1381 aagggaccac ggtcaccgtc tccgcagcct ccaccaaggg cccatcggtc ttccccctgg
     1441 caccctcctc caagagcacc tctgggggca cagcggccct gggctgcctg gtcaaggact
     1501 acttccccga accggtgacg gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca
     1561 ccttcccggc tgtcctacag tcctcaggac tctactccct cagcagcgtg gtgaccgtgc
     1621 cctccagcag cttgggcacc cagacctaca tctgcaacgt gaatcacaag cccagcaaca
     1681 ccaaggtgga caagaaagtt gagcccaaat cttgtgacaa aactagtggc caggccggcc
     1741 agcaccatca ccatcaccat ggcgcatacc cgtacgacgt tccggactac gcttcttagg
     1801 agggtggtgg ctctgagggt ggcggttctg agggtggcgg ctctgaggga ggcggttccg
     1861 gtggtggctc tggttccggt gattttgatt atgaaaagat ggcaaacgct aataaggggg
     1921 ctatgaccga aaatgccgat gaaaacgtgc tacagtctga cgctaaaggc aaacttgatt
     1981 ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg
     2041 ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg
     2101 gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct tccctccctc
     2161 aatcggttga atgtcgccct tttgtctttg gcgctggtaa accatatgaa ttttctattg
     2221 attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct
     2281 ttatgtatgt attttctacg tttgctaaca tactgcgtaa taaggagtct taagctagct
     2341 aattaattta agcggccgca gatctgctct ctgaggagga tctgggaaat tgtaagcgtt
     2401 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2461 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2521 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2581 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2641 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     2701 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     2761 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     2821 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2881 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2941 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3001 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3061 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3121 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3181 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3241 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3301 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3361 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3421 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3481 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3841 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4021 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4081 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4141 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4201 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4261 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4321 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4381 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4441 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4501 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4561 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4621 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4681 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4741 gagcgcagcg agtcagtgag cgaggaagcg ga
//
