LOCUS       sequence-119166-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster 
            blue light-activated Ca2+ channel switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-18"; volume: "6:8021. doi"; pages: " 
            10.1038/ncomms9021"
COMMENT     Sequence Label: mRFP-dmBACCS2
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aagggccctc tagaggtacc ctcgactcaa
      181 actagtccgc tacctccaga gccgccgggc agaccattgt tgttcacaat gtttaagcca
      241 aggcacgtct cgatttgctt ccagcgatgc agtcgttctt gcagctcgtt tgttacatct
      301 ccgagggcat tccgtgcctc aacaatcgac cgatccacat catcaatact ctttccgtgc
      361 gtggaaacga acgcacccac caaacttgac cgtttcttac gcaatttctc acaagcctct
      421 ctggccgact gtagctgctt ctcagccgac gtgcgcttct tctgatgatt cttactttct
      481 agttcatatg tgtattgaag ccatgattgc agattttctg cagttttctt aatcagcatg
      541 actccctctc tctcggcagc atctcggaca tgctcagttc catccaactg aaccccaata
      601 aagtactgga catctccctt ctgatctcgc ataggctgca agtgaaagag gttccagaac
      661 tttttaccac tctttgtata attaatcagc tgaacagtga cctctgtttg gttatctatg
      721 gcatctctaa tttttctcac tgtcgcgcga tcagtttcag gaccttgtag aaacctgcag
      781 tttcttccca aaatttcttc acggctatat tctgtcaact gcaagaaact atcggacgcg
      841 aatataatgg gattatctgg caatcttggg tcagtaatga caaagttctt c
//
