LOCUS       sequence-119173-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for mouse Erg (209-316) with GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pGEX-4T1 Erg (209-316)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(52..57,58..73,74..80)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     93..115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             127..780
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     738..760
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             787..804
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt
       61 aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca
      121 gtattcatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
      181 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      241 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      301 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      361 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      421 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      481 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      541 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      601 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      661 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      721 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      781 tcggatctgg ttccgcgtgg atccccggaa ttcatgcatc tgacttccga tgacgttgat
      841 aaggctttac aaaa
//
