LOCUS       sequence-119179-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for mouse Erg (317-486) with GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pGEX-4T1 Erg (317-486)
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(48..53,54..69,70..76)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    84..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     89..111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
       61 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagtat
      121 tcatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc
      181 ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata
      241 aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg
      301 atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca
      361 acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt
      421 tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag
      481 ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata
      541 aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg
      601 atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta
      661 aaaaacgtat tgaagctatc ccaca
//
