LOCUS       sequence-119182-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus 
            of protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag Pu.1
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1024..1042)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1025..1042)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg
      121 gccgccagtg tgctggaatt caccatggac tacaaggacg acgatgacaa gggaggctta
      181 caggcgtgca aaatggaagg gttttccctc accgcccctc catcggatga cttggttact
      241 tacgattcag agctatacca acgtccaatg catgactact actccttcgt gggcagcgat
      301 ggagaaagcc atagcgatca ctactgggat ttctccgcac accatgtcca caacaacgag
      361 tttgagaact tccctgagaa ccacttcaca gagctgcaga gtgtgcagcc cccgcagcta
      421 cagcagctct atcgccacat ggagctggaa cagatgcacg tcctcgatac tcccatggtg
      481 ccaccccaca ccggcctcag tcaccaggtt tcctacatgc cccggatgtg cttcccttat
      541 caaaccttgt ccccagccca ccagcagagc tcagatgagg aggagggtga gaggcagagc
      601 cctcccctgg aggtgtctga tggagaagct gatggcttgg agcctgggcc aggtcttctg
      661 cacggggaga caggcagcaa gaaaaagatt cgcctgtacc agttcctgct ggacctgctg
      721 cgcagcggcg acatgaagga cagcatctgg tgggtggaca aggacaaagg taccttccag
      781 ttctcgtcca agcacaagga ggcgctggcg caccgctggg gcatccagaa gggcaaccgc
      841 aagaagatga cctaccagaa gatggcgcgc gcgctgcgca actacggcaa gacaggcgag
      901 gtgaagaaag tcaagaagaa gctcacctac cagttcagcg gcgaggtgct gggccgtggg
      961 ggcctggccg agcggcgcct cccgccccac tgatgactcg agcatgcatc tagagggccc
     1021 tattctatag tgtcacctaa atgcta
//
