LOCUS       sequence-119185-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus 
            of protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag Fli1
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    120..344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     333..350
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        370..388
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     370..387
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
       61 gctacagggc gcgtggggat accccctaga gccccagctg gttctttccg cctcagaagc
      121 catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct
      181 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
      241 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
      301 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
      361 agctctagca tttaggtgac actatagaat agggccctct agatgcatgc tcgagctagt
      421 agtagctgcc taagtgtgaa ggcacgtggg tgttaggatg gcgggggaca ctggggtttg
      481 gatagatccc agcagtgggg gaggtccagt attgtgatgc tgctccaaag aagctggagg
      541 aggtgacagg catggaggat gggtgagacg ggacaaagtt caccttctgt tgatgggcat
      601 ggtaggaagg catgtaggag atatcagagg gatacttgta catggatgtc tctgttggat
      661 gtggctgcag ggcctgggca atgccatgga agtcaaactt gtaggcatac cttttgccat
      721 gcactttggt cataatgttt ttgtcatagt agtatcggag ggcccggctc agcttgtcat
      781 aattcatgtt gggcttgctc ttccgctctc cccagcgcct ggccacctca tcagggtccg
      841 tcattttgaa ctccccgttg gtcccctccc aggtgataca gctggcgttg gcgctgtcgg
      901 acagtagttc caggagaaac tgccacagct ggatctgccc actcccaggg tttgctaggc
      961 ggctgctggt tggcccca
//
