LOCUS       sequence-119186-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag Erg
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc gccagcatgg
      121 actacaaaga cgatgacgac aagatccaga ctgtacctga cccagcagct catattaagg
      181 aggccttgtc agttgtgagc gaggaccagt cactatttga gtgtgcctac ggaacgccac
      241 acctggctaa gacagagatg accgcatcct cttccagtga ctatggccag acatccaaga
      301 tgagtcccag agtccctcag caggactggc tgtctcaagc cccagccagg gtcaccatca
      361 agatggagtg caaccctagt caggtgaatg gttccaggaa ctcacctgat gagtgcagtg
      421 tgaacaaagg tgggaagatg gtgggcagcc cggatactgt ggggatgagc tacggcagct
      481 acatggagga gaagcatgtg ccgcctccca atatgaccac aaatgagcgc agagtgatcg
      541 tccctgcaga tcctactctg tggagcacag accatgtccg acagtggctg gagtgggcgg
      601 tgaaagaata tggcctcctc gatgtggacg tcttactatt tcagaatatc gatgggaagg
      661 agctgtgcaa gatgacaaag gatgacttcc agcggctcac gccgagctac aatgccgaca
      721 ttcttctctc acatctccac tacctcagag agactcccct tccacatctg acttccgatg
      781 acgttgataa ggctttacaa aactctccac ggttaatgca tgccagaaac acagggggtg
      841 cagcttttat tttcccaaat acttcagtat atcccgaagc tacgcaaaga attacaacta
      901 ggccagattt accttatgag cctcccagga gatcagcctg gaccggccac agccacctca
      961 cccctcagtc caaagctgct cagccatctc cctctgcagt gcccaaaact gaagaccagc
     1021 gtcctcagtt agatccttac caga
//
