LOCUS       sequence-119202-        4239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL12009
FEATURES             Location/Qualifiers
     source          1..4239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2032..2050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2118..2222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2442..3233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3404..3992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3893..3912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4146..4163
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggaggtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga
      721 cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg
      781 gcaggcggcc tcctcctcct ctcacggcac ggcagctacg ggggattcct ttcccaccgc
      841 tccttcgctt tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt
      901 ccccaacctc gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc
      961 cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc cccccccccc tctctacctt
     1021 ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct gttcatgttt
     1081 gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg gatgcgacct
     1141 gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga
     1201 tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag
     1261 ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat
     1321 cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcta
     1381 gatcggagta gaattctgtt tcaaactacc tggtggattt attaattttg gatctgtatg
     1441 tgtgtgccat acatattcat agttacgaat tgaagatgat ggatggaaat atcgatctag
     1501 gataggtata catgttgatg cgggttttac tgatgcatat acagagatgc tttttgttcg
     1561 cttggttgtg atgatgtggt gtggttgggc ggtcgttcat tcgttctaga tcggagtaga
     1621 atactgtttc aaactacctg gtgtatttat taattttgga actgtatgtg tgtgtcatac
     1681 atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt
     1741 tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc
     1801 taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg
     1861 atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca
     1921 tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt
     1981 tacttctgca ggaatgtgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
     2041 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     2101 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2161 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2221 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2281 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2341 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2401 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     2461 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2521 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2581 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2641 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2701 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2761 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2821 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2881 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2941 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3001 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3061 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3121 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3181 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
     3241 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3301 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3541 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4021 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4081 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4141 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4201 ccgcgcgttg gccgattcat taatcactct gtggtctca
//
