LOCUS       sequence-119232-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: BPK764
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    130..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
       61 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat taatacgact
      121 cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt tgtttaactt
      181 taataaggag atataccatg gataaaaagt attctattgg tttagacatc ggcactaatt
      241 ccgttggatg ggctgtcata accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt
      301 tggggaacac agaccgtcat tcgattaaaa agaatcttat cggtgccctc ctattcgata
      361 gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc tcggagaagg tatacacgtc
      421 gcaagaaccg aatatgttac ttacaagaaa tttttagcaa tgagatggcc aaagttgacg
      481 attctttctt tcaccgtttg gaagagtcct tccttgtcga agaggacaag aaacatgaac
      541 ggcaccccat ctttggaaac atagtagatg aggtggcata tcatgaaaag tacccaacga
      601 tttatcacct cagaaaaaag ctagttgact caactgataa agcggacctg aggttaatct
      661 acttggctct tgcccatatg ataaagttcc gtgggcactt tctcattgag ggtgatctaa
      721 atccggacaa ctcggatgtc gacaaactgt tcatccagtt agtacaaacc tataatcagt
      781 tgtttgaaga gaaccctata aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc
      841 gcctc
//
