LOCUS       sequence-119233-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for Sp-dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP712
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    129..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
      121 actatagggg aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt
      181 aataaggaga tataccatgg ataaaaagta ttctattggt ttagccatcg gcactaattc
      241 cgttggatgg gctgtcataa ccgatgaata caaagtacct tcaaagaaat ttaaggtgtt
      301 ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc tattcgatag
      361 tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt atacacgtcg
      421 caagaaccga atatgttact tacaagaaat ttttagcaat gagatggcca aagttgacga
      481 ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga aacatgaacg
      541 gcaccccatc tttggaaaca tagtagatga ggtggcatat catgaaaagt acccaacgat
      601 ttatcacctc agaaaaaagc tagttgactc aactgataaa gcggacctga ggttaatcta
      661 cttggctctt gcccatatga taaagttccg tgggcacttt ctcattgagg gtgatctaaa
      721 tccggacaac tcggatgtcg acaaactgtt catccagtta gtacaaacct ataatcagtt
      781 gtttgaaga
//
